During this webinar Dr. Sarah Dallas will discuss using live cell imaging in combination with transgenic mice expressing GFP-tagged collagen and fluorescent reporters to visualize bone cell lineages, our work has advanced fundamental understanding of bone extracellular matrix assembly and resorption dynamics. Live cell imaging has revealed the dynamic mechanisms of osteocyte embedding in collagen and their fate after bone resorption. Imaging of osteoclast cell dynamics has revealed novel cell dynamics and real-time mechanisms of bone resorption and allowed us to visualize real time responses to pro- and anti-resorptive agents. Our results show that mature osteoclasts respond dynamically and reversibly to changes in RANKL signaling and highlight the importance of observing osteoclasts and other bone cells in their natural bone environment.
